Standard PCR Enzymes

PCR Enzymes and Kits

Standard PCR                                        l 2013 PCR Enzyme and
                                                              Mastermix Catalog l

ACTaq™ DNA Polymerase

ACTaq™ Blue DNA Polymerase

ACTaq™ Red DNA Polymerase

ACTaq™ DNA Polymerase           P/N:   E2100

Product Description: ACTaq™ DNA Polymerase originates from
 Thermus Aquaticus with molecular
weight of 94KDa. For use in DNA fragment extension amplification
reactions with DNA having dA overhang on 3’ ends; at extension
amplification speed of 1200 bases per minute and optimized between
65-75oC. Optimized for best performance when using dNTP
 concentration
at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

Storage Condition: -20oC. Stable for 12 months in constant freezer
 temperature.

Concentration: 5 Units/μL

Unit Definition: One unit of the amount of enzyme that incorporates
10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ DNA Polymerase, PCR reaction buffer,
10X 25mM MgCl2. (Buffer and MgCl2 concentrations are customizable
upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5%
 NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4,
 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ DNA Polymerase is highly purified. Free
of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.



ACTaq™ Blue DNA Polymerase                      

ACTaq™ Red DNA Polymerase  

P/N:E2100B (blue), E2100R (red)

Product Description: ACTaq™ DNA Polymerase originates from
Thermus Aquaticus with molecular
weight of 94KDa. ACTaq™ Blue or Red differs from ACTaq™
DNA Polymerase in that it contains blue / red dye added to the enzyme
used as tracking and loading dye as no loading buffer is required.

Storage Condition: -20oC. Stable for 12 months in constant freezer
temperature.

Concentration: 1 Unit/μL

Unit Definition: One unit of the amount of enzyme that incorporates
 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ Blue or Red DNA Polymerase,
 PCR reaction buffer, 10X 25mM MgCl2.
(Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA,
0.5% NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4,
 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ Blue or Red DNA Polymerase is highly
 purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.

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PCR test results for ACTaq™ DNA Poly, ACTaq™ Blue DNA Poly
, ACTaq™ Hot-Start DNA Poly, and ACTaq™ Mastermix

1    DNA Marker
2    ACTaq™ DNA Poly
3    ACTaq™ Blue DNA Poly
4    ACTaq™ Hot-Start DNA Poly
5    ACTaq™ Mast